This combination is then incubated at fifty five°C for a person hour. Then the sample is phenol-extracted once with phenol/chloroform/isoamyl alcohol Option and centrifuged. The aqueous layer is taken off to some fresh microcentrifuge tube. The DNA is ethanol-precipitated, re-suspended in buffer, and ethanol-precipitated a next time. Following the pellet https://www.pinterest.com/pin/993958580252568212/